dppD [2019-09-02 13:35:15]

dipeptide [SW|ABC transporter ](ATP-binding protein)

locus: BSU_12950

pI: 7.52

mw: 36.53 kDa

protein length: 335 aa

gene length: 1008 bp

function: uptake of dipeptides

product: dipeptide [SW|ABC transporter ](ATP-binding protein)

essential: no

synonyms: dciAD

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.3|Uptake of peptides]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.4|Utilization of peptides]
• [category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,363,141 1,364,148

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|05752B9C4EA7ACD579D65D028B7DF1861F3840CB|OppD], [protein|325BCDCAB4003204294229B7F9F0A5F353D72B54|AppD]

[SW|Domains]

• ABC transporter domain (aa 7-256) (according to UniProt)

Structure

• [PDB|4FWI] (from Caldanaerobacter subterraneus, 38% identity) [pubmed|23385461]

[SW|Localization]

• attached to the cell membrane (via [protein|062AE5FB4C778178A7F6833CB8BD9D631E461E89|DppB]-[protein|EDF8D2C49FA5552B24135E12524F9035E1E01473|DppC]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|9E922F0006DF9E84A8D5B51E9FC35B669872FF90|dppA]-[gene|062AE5FB4C778178A7F6833CB8BD9D631E461E89|dppB]-[gene|EDF8D2C49FA5552B24135E12524F9035E1E01473|dppC]-[gene|7FA72D949B930A3C7FF0391CC6CCC58EEFCA839A|dppD]-[gene|853C570CFB9382449A20BE77B44B4FE44972AA59|dppE]
  description: [Pubmed|1766371]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1766371], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|7783641], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • repressed by glucose (2.9-fold) [Pubmed|12850135]

• genes: [gene|9E922F0006DF9E84A8D5B51E9FC35B669872FF90|dppA]-[gene|062AE5FB4C778178A7F6833CB8BD9D631E461E89|dppB]-[gene|EDF8D2C49FA5552B24135E12524F9035E1E01473|dppC]-[gene|7FA72D949B930A3C7FF0391CC6CCC58EEFCA839A|dppD]-[gene|853C570CFB9382449A20BE77B44B4FE44972AA59|dppE]-[gene|C4F85F85F0C99A59145961E34B19D3AD03D2BC22|ykfA]-[gene|77EFC47449D8CC17651ED61C41AC605FCEF01270|ykfB]-[gene|D40ECAE51D9E4791C6FAAA25E2E0D0591D9FAA55|ykfC]-[gene|BB94F5388A0D1FF025E5A88A7E10AE7E4C489E5F|ykfD]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

additional information

• [protein|search|translation] is likely to require [protein|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|Efp] due to the presence of several consecutive proline residues [PubMed|23239624,23239623]

Biological materials

Mutant

• BKE12950 ([gene|7FA72D949B930A3C7FF0391CC6CCC58EEFCA839A|dppD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACTGACAGAACTTTTTCCA, downstream forward: _UP4_TCAGGAGATGCGAAGGATTG
• BKK12950 ([gene|7FA72D949B930A3C7FF0391CC6CCC58EEFCA839A|dppD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12950 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GACTGACAGAACTTTTTCCA, downstream forward: _UP4_TCAGGAGATGCGAAGGATTG

References

• 12618455,10092453,1766371,7783641,1766371,23385461